Effect evaluation of Juniperus phoenicea essential oil on a certain biomarkers in mosquito larva

Abstract

As part of the search for effective biological control methods against mosquitoes, an essential oil extracted from the plant of Juniperus phoenicea cultivated in the wilaya of Tebessa (northeastern Algeria) was tested at different lethal concentrations CL25 and CL50 on fourth instar larvae of Culesita longiareolata (L 4 ) collected from untreated areas of Tebessa, under laboratory conditions according to the recommendations of the World Health Organisation . transferase, and catalase), total -s-The effects were examined on two enzymatic biomarkers (Glutathione protein content and the weight growth of Culesita longiareolata at different treatment times, 24h, 48h, and 72h . The determination of the essential oil extraction yield by hydrodistillation of the dried leaves showed a yield of 0.56% . Biomarker results revealed an induction of the detoxification system of Juniperus phoenicea essential oil via an increase in GST and catalase activity compared to controls, as well as a significant decrease in total protein levels with both CL25 and CL50 concentrations at different treatment periods . Juniperus phoenicea essential oil (LC25,LC50) affected the weight growth of the mosquito species with a significant reduction in the body weight of C. longiareolata larvae compared to controls at different treatment periods . Research into natural products extracted from plants capable of controlling populations of Culiseta longiareolata is very important as it can help to reduce the spread of the spread of the disease from this -vector. According to the data from these studies, Juniperus phoenicea essential oil can be used as a bio insecticide and as an alternative to synthetic products in mosquito control