Antioxydant activity and combined toxic effect of Ruta graveolens and Ruta montana essential oils on Culiseta longiareolata larvae

Abstract

Several studies have been concerned for several years with the various therapeutic and anti-insect activities of medical plants because they contain the source of bioactive natural compounds. In this context, we conducted this study, which was aimed at laboratory research on the antioxidant activities of essential oils R graveolens and R montana , and Evaluate the anti insect activity of both of the plant's essential oils against the most abundant Culiseta longiareolata larvae in Tebessa, to determine the common toxic effect of both essential oils. The base oil was obtained by distilling using a clevenger-type gives an essential oil R montana and R .graveolens with a yield 0,55% and 0,48.% of dry matter from the aerial part of the two plants respectively. Evaluate antioxidant activity. Using a technique DPPH Test , R graveolens EO inhibits oxidative activity with a percentage ranging from 20.26% (at 0.1mg/mL) up to 37.01% (at 1.6mg/mL). R. montana EO inhibits oxidative activity with a percentage ranging from 18.49% (at 0.1mg/mL) up to 43.65%. Determine the lethal concentrations ( CL25 and CL50and CL90) , Evaluate toxicity effect the combined oils essential using three different mixtures ( 75 %R graveolens and 25% R montana ) ,(50 % R graveolens and 50% R montana ) , (25% R graveolens and 75 % R montana) Using several concentrations on the fourth phase larvae Culiseta longiareolata in laborator conditions as directed by the World Health Organization ,Toxicity tests that these oils possess pesticide properties with the relationship between dose and response. Our results confirm that essential oils enjoy biological activity of insecticides and antioxidants and justify their effectiveness as an alternative to harmful chemicals.