Toxicity of Fe3O4 nanoparticles on oxidative stress status, stromal enzymes and mitochondrial respiration and swelling of Oryctolagus cuniculus brain cortex

Abstract

Fe3O4 nanoparticles are the most widely used metal oxide nanoparticles especially, in biomedical applications. Although, nanoparticles can enter to the different organs, little is known so far on the neurotoxic potential and oxidative stress of Fe3O4. Here the understanding of the effect of Fe3O4 nanoparticles on the general Redox state of rabbit brain and the effect on mitochondrial swelling and respiration were assessed. Fe3O4 resulted in increase of brain markers, lipid peroxidation, protein and ROS formation. Mitochondrial enzymes and swelling were elevated with decreased respiration level. Caspase 3 activity and TNF-α level were also increased. Finally, our study suggested that the mitochondrial disease and dysfunction with elevated oxidative stress in rabbit brain treated with 200 and 300 μg/kg per Os is the original of neurotoxicity and maybe the original cause of neurodegenerative disease.