Extraction of calcium carbonate and collagen from egg shells

Abstract

Around the world, large quantities of eggs are consumed in a variety of ways, producing enormous numbers of eggshells. These eggshells may wind up in landfills, rivers, or coastal waters, which may have an adverse effect on people's health, contaminate water supplies, or otherwise harm the environment. Additionally, in recent years, particular attention has been paid to industrial sectors that pollute the environment. Eggshells have some organic membranes and contain a lot of CaCO3, although they are typically regarded as garbage. ES comprise roughly 95% calcium carbonate, and the ES membrane's fibrils are primarily made of proteins, around 10% of which are collagen (types I, V, and X). Due to the versatility of both calcium carbonate and cllagen in many fieldssuch as medicine, pharmaceutic, alimentation and cosmetic. In this study, we used waste eggshell as raw material to extract calcium carbonate and collagen as bio and natural products, and in this study we gave an attempt to reduce waste eggshell, including environmental pollution, in order to preserve our health and achieve the zero waste rule. 1 kg of eggshell collected from 2 different restaurants, we used just 100g of the extern membrane to extract CaCO3 and 50g from the inner membrane to extract collagen. The eggshells were cleaned and sterilized, both membranes were separated manually. The outer membrane was ground and mixed with 10% of acetic acid in order to extract CaCO3. The inner membrane went through two stages, the first one is the pre-treatment to remove impurity such as soluble non-collagenous compound, lipids, pigments and aftertaste. The second one is the extraction step of collagen by 0.5M of citric or acetic acid. In this study, the presence of calcium carbonate content in ES was determined by back titration. The result of this study was high yield of pure material of CaCO3 and collagen, the rest of the details and information you will get through the upcoming papers