Résumé:
Actinomycetes are gram-positive filamentous bacteria that receive great attention as producers of
biomolecules and are distributed in various environmental media.
The goal of this work revolves around searching for biomolecules produced by actinomycetes that
love harsh and dry conditions and studying their physical and chemical properties. Through this
discussion. We are interested in proving that 7 of the Actinomyces strains have the ability to produce
antibiotics against 4 targe bacterial strains of klebsilla, staphilococcus and bacillus and are capable of
producing antifungals against 5 strains of yeasts of candida, saccharomycs, rhodotorula and
kluveromycs and against 6 strains. of the filamentous fungi of the type Fusarium, Lichthermia,
Corylinifica, Rhizopus and Ac.fleww. We used the double layer technique in ISP2 culture media in
only 10 days and an agar cylinder on different days (7,10) in different culture media ISP2, GYEA.
Its production of special enzymes was studied, as well as the physiological conditions of pH. Salinity
and temperature.
Through the results obtained, we found that the majority of the 07 isolated strains present
antibacterial and antifungal activity, with diameters of inhibition reaching 74.5 mm against bacteria,
77.5 mm against yeasts, and 73.7 mm against fungi. Based on the results of physiological conditions,
all isolated strains grow at 25°C and are salt-loving. As for the study of the metabolic profiles of these
strains, it showed that these strains have an important diversity in enzymatic activity.
In conclusion, actinomycetes can be considered specialized in producing important biomolecules in
the medical field, distinguished by their metabolic capacity and their distribution in various media.
