Lavidical activity of Ziziphus lotus hydro-ethanolic extract against mosquito species : morphometric and biochemical alteration

Abstract

This study aims to evaluate the larvicidal activity of the hydroethanolic extract of Ziziphus lotus against Culiseta longiareolata mosquito larvae, focusing on morphometric and biochemical changes.The extraction yield of the aerial part of Ziziphus lotus was 24.17%. Fourth-instar larvae were exposed to sublethal concentrations (LC25 and LC50) of this plant over different time periods (24 hours, 48 hours, and 72 hours). The study included morphometric assessments (neck diameter and weight) and biochemical assessments (total protein, carbohydrates, and lipids). The results showed that Ziziphus lotus extracts induce statistically significant changes in both the morphology and biochemistry of the larvae. Therefore, these data suggest that the hydroethanolic extracts of Ziziphus lotus may represent an environmentally safe alternative for controlling mosquito populations.